Comparison of Inflammatory Responses in Mouse Lungs Exposed to Atranones A And C fromStachybotrys Chartarum
Abstract
1239 Journal of Toxicology and Environmental Health, Part A, 69:1239–1251, 2006 Copyright© Taylor & Francis Group, LLC ISSN: 1528–7394 print / 1087–2620 online DOI: 10.1080/15287390500360307 COMPARISON OF INFLAMMATORY RESPONSES IN MOUSE LUNGS EXPOSED TO ATRANONES A AND C FROM STACHYBOTRYS CHARTARUM Thomas G. Rand1, J. Flemming1, J. David Miller2, Taiwo O. Womiloju2 1Department of Biology, Saint Mary’s University, Halifax, Nova Scotia, Canada 2Department of Chemistry, Carleton University, Ottawal, Ontario, Canada Stachybotrys chartarum isolates can be separated into two distinct chemotypes based on the toxins they produce. One chemotype produces macrocyclic trichothecenes; the other produces atranones (and sometimes simple trichothecenes, e.g., trichodermol and trichodermin). Studies using in vivo models of lung disease revealed that exposure to spores of the atranone producing S. chartarum isolates led to a variety of immunotoxic, inflammatory, and other pathological changes. However, it is unclear from these studies what role the pure atranone toxins seques- tered in spores of these isolates exert on lung disease onset. This study examined dose-response (0.2, 1.0, 2.0, 5.0, or 20 mg atranone/animal) and time-course (3, 6, 24, and 48 h postinstilla- tion [PI]) relationships associated with inflammatory cell and proinflammatory chemokine/cytok- ine responses in mouse lungs intratracheally instilled with two pure atranones (either A or C) isolated from S. chartarum. High doses (2.0 to 20 mg toxin/animal) of atranone A and C induced significant inflammatory responses manifested as differentially elevated macrophage, neutrophil, macrophage inflammatory protein (MIP)-2, tumor necrosis factor (TNF) and interleukin (IL)-6 concentrations in the bronchioalveolar lavage fluid (BALF) of intratracheally exposed mice. Com- pared to controls, BALF macrophage and neutrophil numbers were increased to significant levels from 6 to 48 h (PI). Except for macrophage numbers in atranone
Key Biomarkers
Symptom Clusters
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- Effects of low molecular weight fungal compounds on inflammatory gene transcription and expression in mouse alveolar macrophagesChemico-Biological Interactions · 2011
- Inflammatory and cytotoxic responses in mouse lungs exposed to purified toxins from building isolated Penicillium brevicompactum Dierckx and P. chrysogenum ThomToxicological Sciences · 2005
- In Vitro Metabolism of Phenylspirodrimanes Derived from the Indoor Fungus StachybotrysToxins · 2022
- Inflammation-associated gene transcription and expression in mouse lungs induced by low molecular weight compounds from fungi from the built environmentChemico-Biological Interactions · 2010
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