Impaired host defense to infection and Toll-like receptor 2-independent killing ofBorrelia burgdorfericlinical isolates in TLR2-deficient C3H/HeJ mice
Abstract
To investigate the role of Toll-like receptor 2 (TLR2)-mediated signaling in host innate defense and development of Lyme disease, the pathogenicity of Borrelia burgdorferi sensu stricto clinical isolates representing two distinct genotypes (RST1 and RST3A) was assessed in TLR2(-/-) C3H/HeJ mice. All TLR2(-/-) mice infected with a B. burgdorferi RST1 isolate developed severe arthritis. The numbers of spirochetes in heart, joint and ear biopsy specimens were significantly higher in TLR2(-/-) mice than in wild-type mice similarly infected as determined by real-time quantitative polymerase chain reaction. Interestingly, despite the higher spirochete levels in heart tissues, milder carditis was observed in TLR2(-/-) than in wild-type mice infected with this RST1 isolate (P=0.02). By contrast, no positive cultures were obtained from any of the blood and tissue specimens from TLR2(-/-) mice inoculated with two RST3A clinical isolates. The data suggest that there is impaired host innate defense against infection and TLR2-independent killing of B. burgdorferi clinical isolates in TLR2-deficient C3H/HeJ mice.
Cited By (3)
- Borrelia burgdorferiInduces TLR1 and TLR2 in Human Microglia and Peripheral Blood Monocytes but Differentially Regulates HLA-Class II ExpressionJournal of Neuropathology & Experimental Neurology · 2006
- Activation of innate host defense mechanisms by BorreliaEuropean Cytokine Network · 2012
- T cell infiltration is associated with increased Lyme arthritis in TLR2−/−miceFEMS Immunology & Medical Microbiology · 2008
References (4)
- Cutting Edge: Inflammatory Signaling by Borrelia burgdorferi Lipoproteins Is Mediated by Toll-Like Receptor 2The Journal of Immunology · 1999
- Toll-Like Receptor 2 Is Required for Innate, But Not Acquired, Host Defense to Borrelia burgdorferiThe Journal of Immunology · 2002
- Genetic Diversity of Borrelia burgdorferi in Lyme Disease Patients as Determined by Culture versus Direct PCR with Clinical SpecimensJournal of Clinical Microbiology · 1999
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