Optimizing a GC-MS method for screening of Stachybotrysmycotoxins in indoor environments

Abstract

-7 POPULARVETENSKAPLIG SAMMANFATTNING - 8 ABBREVIATIONS -10 INTRODUCTION -13 INDOOR AIR QUALITY AND HEALTH -13 MICROORGANISMS IN WATER DAMAGED INDOOR ENVIRONMENTS -15 EXPOSURE ASSESSMENT METHODS FOR MOLD -16 MOLDS AND THEIR BIOLOGICALLY ACTIVE METABOLITES - 18 DETECTION OF MYCOTOXINS -26 Mass Spectrometty -27 GC-MS -29 HPLC-MS -31 MS detection of mycotoxins - 33 AIMS OF THE STUDY -35 MATERIAL AND METHODS - 37 SAMPLES -37 SAMPLING AND CULTIVATION - 38 MICROSCOPY -38 QPCR -39 CHEMICAL EXPERIMENTS -39 Extraction -39 Purification and hj'drolysis -40 Derivati^ation (for GC-MS) - 41 GC-MS -42 HPLC-MS -45 SUMMARY OF RESULTS - 49 MASS SPECTROMETRY - 49 OVERVIEW OF ALL ANALYZED SAMPLES -50 ADDITIONAL COMMENTS - 52 DISCUSSION -53 MOLD IN BUILDINGS - GUIDELINES AND REGULATIONS - 53 THIS STUDY IN CONTEXT - 56 CONCLUSIONS -59 ACKNOWLEDGEMENTS -61 REFERENCES -63 PAPERSI-IV -81