Immune Complexes from Serum of Patients with Lyme Disease ContainBorrelia burgdorferiAntigen and Antigen‐Specific Antibodies: Potential Use for Improved Testing
Abstract
We report sequestration of specific IgM anti-Borrelia burgdorferi (Bb) and Bb antigens within immune complexes (ICs) isolated from serum of patients with Lyme disease (LD). The relative enrichment in specific IgM measured by ELISA was apparent, even after correcting for differences in total IgM concentration in serum versus ICs. Immunoblot demonstrated that ICs contained antibodies against specific Bb proteins, whereas reactivity was absent or significantly lessened in unprocessed serum. This is the first study to show ICs containing Bb antigen identified by immunoblot with anti-Bb monoclonal antibody. ICs may be a useful source of antigen and antibody for development of more-accurate testing for LD.
Key Biomarkers
Cited By (2)
- Use of Serum Immune Complexes in a New Test That Accurately Confirms Early Lyme Disease and Active Infection with Borrelia burgdorferiJournal of Clinical Microbiology · 2001
- New method for detection of Borrelia burgdorferi antigen complexed to antibody in seronegative Lyme diseaseJournal of Immunological Methods · 2001
References (4)
- Association of treatment-resistant chronic Lyme arthritis with HLA-DR4 and antibody reactivity to OspA and OspB of Borrelia burgdorferiInfection and Immunity · 1993
- Cerebrospinal fluid immune complexes in patients exposed to Borrelia burgdorferi: Detection of Borrelia‐specific and ‐nonspecific complexesAnnals of Neurology · 1990
- Sequestration of antibody to Borrelia burgdorferi in immune complexes in seronegative Lyme diseaseThe Lancet · 1990
- Detection and preliminary characterization of circulating immune complexes in patients with Lyme diseaseMedical Microbiology and Immunology · 1997
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