Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease
Abstract
The sensitivity and specificity of an indirect immunofluorescence assay (IFA) and ELISA for Lyme disease were estimated. Sera from patients with Lyme disease, patients with other infections, and healthy individuals were examined. Significant cross-reactivity occurred only with sera from patients with syphilis, yaws, and pinta . All tested sera from patients with Lyme disease, however, gave negative results in the rapid reagin screening test and the microhemagglutination assay for antibodies to Treponema pallidum confirmatory for syphilis. When sera from patients with treponemal diseases were excluded from the analysis, the IFA and ELISA were highly specific, having 97% and 100% reliability, respectively. Sensitivity of both tests varied with the stage of disease but was 100% for both tests during complicated Lyme disease. The results indicate that both tests are highly specific and sensitive for complicated Lyme disease but relatively insensitive for patients with erythema chronicum migrans alone.
Cited By (7)
- Persistence of serum antibodies to Borrelia burgdorferi in patients treated for Lyme diseaseClinical Infectious Diseases · 1992
- TREATMENT OF LATE LYME BORRELIOSIS—RANDOMISED COMPARISON OF CEFTRIAXONE AND PENICILLINThe Lancet · 1988
- The Prevalence and Incidence of Clinical and Asymptomatic Lyme Borreliosis in a Population at RiskThe Journal of Infectious Diseases · 1991
- Ceftriaxone Compared with Doxycycline for the Treatment of Acute Disseminated Lyme DiseaseNew England Journal of Medicine · 1997
- The important and diverse roles of antibodies in the host response to Borrelia infectionsCurrent topics in microbiology and immunology · 2008
- Interlaboratory comparison of test results for detection of Lyme disease by 516 participants in the Wisconsin State Laboratory of Hygiene/College of American Pathologists Proficiency Testing ProgramJournal of Clinical Microbiology · 1997
- Performance of 45 Laboratories Participating in a Proficiency Testing Program for Lyme Disease Serology
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