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Effect of Moxifloxacin on Production ofProinflammatory Cytokines from Human Peripheral BloodMononuclearCells

Choi JH, Song MJ, Kim SH +4 more2003Antimicrobial Agents and ChemotherapyIn Vitro
10.1128/AAC.47.12.3704-3707.2003PubMedFree full text
Immune/Innate
Bacterial Endotoxins

Abstract

The effects of moxifloxacin, a new methoxyfluoroquinolone, on the production of proinflammatory cytokines from human peripheral blood mononuclear cells (PBMCs) were evaluated. Moxifloxacin inhibited the production of tumor necrosis factor alpha (TNF-alpha) and/or interleukin-6 (IL-6) by PBMCs stimulated with lipopolysaccharide (LPS), lipoteichoic acid (LTA), and heat-killed bacteria in a concentration-dependent manner without cytotoxic effects. The addition of moxifloxacin reduced the population of cells positive for CD-14 and TNF-alpha and for CD-14 and IL-6 among the LPS- or LTA-stimulated PBMCs. By Western blot analysis, moxifloxacin pretreatment reduced the degradation of IkappaBalpha in LPS-stimulated PBMCs. In conclusion, moxifloxacin could interfere with NF-kappaB activation by inhibiting the degradation of IkappaBalpha and reduce the levels of production of proinflammatory cytokines.

Key Biomarkers

CD-14IkappaBalphaIL-6 (Interleukin-6)NF-kappaBTNF-alpha (Tumor Necrosis Factor Alpha)

References (2)

  • Clarithromycin Inhibits NF-κB Activation in Human Peripheral Blood Mononuclear Cells and Pulmonary Epithelial CellsAntimicrobial Agents and Chemotherapy · 2001
  • In vitro effects of ciprofloxacin and roxithromycin on apoptosis of jurkat T lymphocytesAntimicrobial Agents and Chemotherapy · 2003

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