Fluorescence assay for studying the ability of macrolides to induce production of ribosomal methylase

Clarebout G; Leclercq R

doi:10.1128/aac.46.7.2269-2272.2002

Fluorescence assay for studying the ability of macrolides to induce production of ribosomal methylase

Clarebout G, Leclercq R2002Antimicrobial Agents and ChemotherapyJournal Article

10.1128/aac.46.7.2269-2272.2002 PubMed Free full text

Abstract

A screening assay to test the inducing capacity of macrolides by fusing the attenuator of the inducible erm (B) gene from Streptococcus pneumoniae HM28 with the gfpmut1 gene has been designed. Fluorescence was detected under UV light around disks impregnated with inducer macrolides (erythromycin or azithromycin) but not with noninducer ketolides. Induction could be quantified by fluorometry.

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