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A novel qPCR based-method for detection and quantification of three recurrent species of Penicillium isolated from bioaerosols in mold-damaged homes

Antoine Géry, Antoine Delanoë, Natacha Heutte +3 more2021Journal of Microbiological MethodsJournal Article
10.1016/j.mimet.2021.106236PubMedFree full text
Indoor Mold (Stachybotrys, Aspergillus, etc.)

Abstract

Fungal contamination of indoor environments can cause respiratory diseases and induce damages to building materials. Among the fungal species found in mold-damaged homes, Penicillium brevicompactum, P. chrysogenum and P. crustosum can be considered as recurrent strains. In this study, we therefore propose a rapid and novel qPCR-based method in order to allow the monitoring of these three fungal species. The method developed allows the quantification of the target DNA of these three Penicillium species with a limit of quantification of 0.01 ng/μL without significant difference with spectrophotometry quantification assay for DNA concentrations between 5 and 100 ng/μL. This technique also enables the rapid detection of these three species in complex mixtures of DNA extracted from 15 bioaerosols collected in mold-damaged homes and previously cultured on agar plate. This new sensitive and specific qPCR technique can thus be easily integrated into bioaerosol studies.

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  • Associations between Fungal Species and Water-Damaged Building MaterialsApplied and Environmental Microbiology · 2011

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